Indian Journal of Nephrology About us |  Subscription |  e-Alerts  | Feedback | Login   
  Print this page Email this page   Small font sizeDefault font sizeIncrease font size
 Home | Current Issue | Archives| Ahead of print | Search |Instructions |  Editorial Board  

Users Online:208

Official publication of the Indian Society of Nephrology
  Search
 
 ~ Next article
 ~ Previous article 
 ~ Table of Contents
  
 ~  Similar in PUBMED
 ~  Search Pubmed for
 ~  Search in Google Scholar for
 ~Related articles
 ~  Article in PDF (134 KB)
 ~  Citation Manager
 ~  Access Statistics
 ~  Reader Comments
 ~  Email Alert *
 ~  Add to My List *
* Registration required (free)  

 
   Abstract
   Introduction
   Materials and Me...
   Results
   Discussion
   Conclusion
   References
   Article Tables

 Article Access Statistics
    Viewed1731    
    Printed163    
    Emailed2    
    PDF Downloaded155    
    Comments [Add]    
    Cited by others 2    

Recommend this journal

 


 
Previous articleNext article
ORIGINAL ARTICLE
Year : 2010  |  Volume : 20  |  Issue : 4  |  Page : 190-192
 

Ten year experience of pediatric kidney biopsies from a single center in Pakistan


Department of Medicine, Aga Khan University Hospital, Stadium Road, Karachi - 74800, Pakistan

Date of Web Publication1-Dec-2010

Correspondence Address:
A Absar
Department of Medicine, Nephrology Section FOB Building, First Floor, Aga Khan University Hospital, Stadium Road, Karachi 74800
Pakistan
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0971-4065.73446

Rights and Permissions

  Abstract 

There are many established registries of kidney biopsies around the world. In addition, there are several reports available in literature from many countries on pediatric kidney biopsy. This study was done to determine the indications and pathological patterns of kidney biopsies of children referred to our hospital, and compare our data with the data available from other countries. This is a cross-sectional study of pediatric kidney biopsies over a 10-year period, from January 1997 to December 2006. All biopsies were done in Aga Khan University Hospital, Karachi, Pakistan. Age range was from 1 to 14 years. Data were analyzed for indications and histopathological diagnosis. A total of 54 kidney biopsies were included in the initial analysis. Here 13 samples were excluded and final analysis was done on the remaining 41 samples. The most common indication of kidney biopsy was nephrotic syndrome in 25 samples (61%). The most common histopathology was minimal change disease in 15 (37%), followed by focal segmental glomerulosclerosis in 5 (12%) of the biopsies.


Keywords: Kidney, biopsy, pediatric, indications, pathology


How to cite this article:
Absar A, Diamond M, Sonia Y, Arshalooz R, Safia A, Waqar K, Shahid P. Ten year experience of pediatric kidney biopsies from a single center in Pakistan. Indian J Nephrol 2010;20:190-2

How to cite this URL:
Absar A, Diamond M, Sonia Y, Arshalooz R, Safia A, Waqar K, Shahid P. Ten year experience of pediatric kidney biopsies from a single center in Pakistan. Indian J Nephrol [serial online] 2010 [cited 2017 Oct 20];20:190-2. Available from: http://www.indianjnephrol.org/text.asp?2010/20/4/190/73446



  Introduction Top


A kidney biopsy is relatively uncommon in children compared with adults. A literature review indicates that most of the published reports deal with adult kidney biopsy. This difference is due to the fact that nephrotic syndrome in children is assumed to have minimal disease change, and steroid therapy is instituted first without biopsy. [1],[2] Kidney biopsy in nephrotics is done only when they have steroid-resistant or steroid-dependent nephrotic syndrome. [3] Now, with the availability of automated triggered biopsy gun, kidney biopsy is a safe procedure and usually not an issue in decision-making. [4]

There are several published reports on pediatric kidney biopsy from various countries. However, a Medline search revealed that there is no published report from Pakistan on this subject. We did this study to determine the pattern of pathological diagnosis and to identify the indications of renal biopsies in children referred to our institution.


  Materials and Methods Top


This is a cross-sectional study of pediatric kidney biopsies performed over a period of 10 years between January 1997 and December 2006. All biopsies were done in Aga Khan University Hospital, Karachi, which is a tertiary care medical center located in a large cosmopolitan city of Pakistan. All biopsies performed in Aga Khan University Hospital in children 1-14 years of age were collected.

We excluded biopsies form and there with transplant kidney, tumor, inconclusive results

Minimum workup of the patients included the following: 24-h urine protein or spot urine protein/creatinine ratio; urine microscopy; blood urea nitrogen and serum creatinine; and renal sonogram. Additional tests were performed when indicated.

All biopsies were done by a trained nephrologist or an invasive radiologist. Either the automated biopsy gun or a traditional tru-cut biopsy needle was used. Histopathological evaluation of the biopsy specimens was done by light microscopy and immunofluorescence. Electron microscopy was not available. Biopsies were stained with hematoxylin-eosin and periodic acid schiff. Immunoflourescence staining was done with antibodies against IgG, IgM, IgA, C 3 , and other markers if indicated. All specimens were reviewed by two qualified pathologists. The data were stored and analyzed by statistical package for social sciences (SPSS) version 16.


  Results Top


Total 54 kidney biopsies were included in the initial analysis. After excluding 13 biopsy samples, there were 41 biopsies remaining for final analysis. There were 29 male (72.5%) and 11 female subjects (27.5%). Mean age was 7.6 ΁ 3.3 years. Median age was 9 years. Age range was 1-14 years.

The analysis showed that the most common indication of biopsy was nephrotic syndrome in 25 (61%) patients. Other indications were non-nephrotic proteinuria, acute renal failure, chronic renal failure, lupus, and oliguria [Table 1].
Table 1: Indications of kidney biopsy

Click here to view


Among 25 nephrotic patients, 8 (32%) were steroid dependent and 17 (68%) were steroid resistant. Steroid dependence refers to the relapse on withdrawal of steroids and requirement of steroids to maintain the remission. Steroid resistance refers to little or no reduction in proteinuria after 12-16 weeks of adequate therapy. [5]

The most common histopathology was minimal change disease (MCD) in 15 (37%) patients. It was followed by focal segmental glomerulosclerosis (FSGS) in 5 (12%) patients. Membrano-proliferative glomerulonephritis (MPGN) was noted in 4 (10%) of samples [Table 2].
Table 2: Pathological diagnosis of kidney biopsy

Click here to view



  Discussion Top


It is the first report of pediatric renal biopsy from Pakistan. This study provides information about indications of kidney biopsy as well as a pattern of histopathology in children. The most common indication of renal biopsy was nephrotic syndrome. Steroid-resistant nephrotics were twice of steroid-dependent nephrotics in number.

In our study, the most common histopathological diagnosis, irrespective of the indication of biopsy, was MCD. The second most common pathology was FSGS and then MPGN.

Results from some of the representative studies from other geographical locations are presented in [Table 3]. The three most common histopathologies in NS stand out to be MCD, FSGS, and MPGN, not necessarily in the same order. [6],[7],[8],[9],[10],[11],[12],[13],[14],[15] Probably, lower the threshold of doing kidney biopsy, greater the chance of picking up cases of MCD.
Table 3: Summary of results of studies from various countries

Click here to view


There is ongoing debate regarding the practice patterns of doing pediatric kidney biopsy. Filler and associates form Canada reviewed 17 years data of pediatric renal biopsy. [16] They concluded that in spite of reports of increasing incidence of FSGS, there is no reason to change the initial therapy and current indications to perform renal biopsy in children. Others recommended to modify the current practice of pediatric kidney biopsy to minimize the likelihood of kidney biopsy with MCD. [15] Webb and associates reviewed childhood steroid-sensitive nephrotic syndrome. They concluded that prebiopsy clinical courses do not predict the histological diagnosis. [17]

We recommend to keep low threshold for the procedure, especially because it is now a very safe procedure. Biopsy will help in making early diagnosis and instituting the right doses of steroids or other immunosuppressive medications promptly according to the guidelines published by the Indian Pediatric Nephrology Group. [5]


  Conclusion Top


Our study is an important contribution to the epidemiology of pediatric renal diseases in South-East Asia. We conclude that MCD is the most common pathology and nephrotic syndrome is the most common indication of biopsy in our children. There is an urgent need of central registry of pediatric kidney biopsy in our country to better understand the pattern of renal disease among children.

 
  References Top

1.Hudson EM, McKnight JF, Willis NS. Corticosteroid therapy for nephrotic syndrome in children. Cochrane Database Syst Rev 2005;1:CD001533.   Back to cited text no. 1
    
2.Brodehl J. The treatment of minimal change nephrotic syndrome: lessons learned from multicentre co-operative studies. Eur J Pediatr 1991;150:380-7.  Back to cited text no. 2
[PUBMED]    
3.McKinney PA, Feltbower RG, Brocklebank JT, Fitzpatrick MM. Time trends and ethnic patterns of childhood nephrotic syndrome in Yorkshire, UK. Pediatr Nephrol 2001;16:1040-4.  Back to cited text no. 3
[PUBMED]  [FULLTEXT]  
4.Feneberg R, Schaefer F, Zieger B, Waldherr R, Mehls O, Schδrer K. Percutaneous renal biopsy in children: a 27-year experience. Nephron 1998;79:438-46.  Back to cited text no. 4
    
5.Bagga A. Revised guidelines for management of steroid sensitive nephrotic syndrome. Indian J Neph 2008;18:31-9.   Back to cited text no. 5
    
6.Coppo R, Gianoglio B, Porcellini MG, Maringhini S. Frequency of renal diseases and clinical indications for renal biopsy in children (report of the Italian National Registry of Renal Biopsies in Children). Group of Renal Immunopathology of the Italian Society of Pediatric Nephrology and Group of Renal Immunopathology of the Italian Society of Nephrology. Nephrol Dial Transplant 1998;13:293-7.  Back to cited text no. 6
[PUBMED]    
7.al-Rasheed SA, al-Mugeiren MM, al-Salloum AA, al-Sohaibani MO. Childhood renal diseases in Saudi Arabia: A clinicopathological study of 167 cases. Int Urol Nephrol 1996;28:607-13.  Back to cited text no. 7
[PUBMED]    
8.Bircan Z, Yavuz Yilmaz A, Katar S, Vitrinel A, Yildirim M. Childhood idiopathic nephrotic syndrome in Turkey. Pediatr Int 2002;44:608-11.  Back to cited text no. 8
[PUBMED]  [FULLTEXT]  
9.Bazina M, Glavina-Durdov M, Scukaenec-Spoljar M, Bazina A, Vukojevic K, Ljutic D, et al. Epidemiology of Renal Disease in Children in the region of Southern Croatia: A 10 Year Review of Regional Renal Biopsy Database. Med Sci Monit 2008;134:172-6.  Back to cited text no. 9
    
10.Madani A, Fahimi D, Esfehani ST, Mohsseni P, Atayee N, Ahmadi M, et al. Glomerular Disease In Iranina Children: Clinicopathological Correlations. Pediatr Nephrol 2003;18:925-8.  Back to cited text no. 10
[PUBMED]  [FULLTEXT]  
11.Choi IJ, Jeong HJ, Han DS, Lee JS, Choi KH, Kang SW, Ha SK, et al. An analysis of 4,514 cases of renal biopsy in Korea. Yonsei Med J 2001;42:247-54.  Back to cited text no. 11
[PUBMED]  [FULLTEXT]  
12.Nammalwar BR, Vijayakumar M, Prahlad N. Experience of renal biopsy in children with nephrotic syndrome. Pediatr Nephrol 2006;21:286-8.  Back to cited text no. 12
[PUBMED]  [FULLTEXT]  
13.Srivsata T, Simmon SD, Alon US. High incidence of Focal Segmental Glomerulo- sclerosis in Nephrotic Syndrome of childhood. Pediat Nephrol 1999;13:13-8.  Back to cited text no. 13
    
14.Gulati S, Sharma AP, Sharma RK, Gupta A, Gupta RK. Do current recommendations for kidney biopsy in nephrotic syndrome need modifications? Pediatr Nephrol 2002;17:404-08.  Back to cited text no. 14
[PUBMED]  [FULLTEXT]  
15.Bonilla-Felix M, Parra C, Dajani T, Ferris M, Swinford RD, Portman RJ, et al. Changing patterns in the histopathology of idiopathic nephrotic syndrome in children. Kidney Int 1999;55:1885-90.   Back to cited text no. 15
[PUBMED]  [FULLTEXT]  
16.Filler G, Young E, Geier P, Carpenter B, Drukker A, Feber J. Is there really an increase in non-minimal change nephrotic syndrome in children? Am J Kidney Dis 2003;42:1107-13.  Back to cited text no. 16
[PUBMED]  [FULLTEXT]  
17.Webb NJ, Lewis M, Iqbal J, Smart PJ, London M, Postlethwaite RJ. Childhood steroid sensitive nephrotic syndrome. Does the histology matters. Am J Kidney Dis 1996;27:484-8.  Back to cited text no. 17
    



 
 
    Tables

  [Table 1], [Table 2], [Table 3]

This article has been cited by
1 Native kidney biopsies in Armenian and Swiss children: high prevalence of amyloidosis in Yerevan and of IgA nephropathy in Zurich
Guido F. Laube,Ashot Sarkissian,Helen Nazaryan,Giuseppina Spartą,Armen Sanamyan,Ara Babloyan,Ernst Leumann,Ariana Gaspert
Virchows Archiv. 2014;
[Pubmed] | [DOI]
2 Comment on "ten-year experience of pediatric renal biopsies from a single center in Pakistan"
Mubarak, M.
Indian Journal of Nephrology. 2012; 22(1): 66
[Pubmed]



 

Top
Print this article  Email this article
Previous articleNext article

    

© Indian Journal of Nephrology
Published by Wolters Kluwer - Medknow
Online since 20th Sept '07