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Year : 2020  |  Volume : 30  |  Issue : 1  |  Page : 8--13

Immunoperoxidase and immunofluorescence on formalin-fixed, paraffin-embedded tissue sections versus immunofluorescence on frozen sections in the assessment of renal biopsies

Nasar Yousuf Alwahaibi1, Rajaa Mohammed Alsidiri1, Thuraiya Amer Alsinawi1, Wafa Hamed Almalki1, Shadia Said Alsinawi2, Marwa Abdullah Alriyami2 
1 Department of Allied Health Sciences, College of Medicine and Health Sciences, Sultan Qaboos University, Sultanate of Oman
2 Department of Pathology, College of Medicine and Health Sciences, Sultan Qaboos University, Sultanate of Oman

Correspondence Address:
Dr. Nasar Yousuf Alwahaibi
Department of Allied Health Sciences, College of Medicine and Health Sciences, Sultan Qaboos University, P.O. Box 35 Postal Code 123, Muscat
Sultanate of Oman

Introduction: There are few published studies comparing immunofluorescence on formalin-fixed, paraffin-embedded (FFPE) tissue sections (IF-P) and immunoperoxidase on FFPE tissue sections (IP-P) with immunofluorescence on frozen sections (IF-F) for evaluation of renal diseases. Also, the accuracy for each method differs greatly. The aim of this study was to evaluate IF-P and IP-P as an alternative to IF-F (gold standard method) in the diagnosis of renal biopsies specimens. Methods: In all, 101 renal biopsies were subjected to IF-P, IP-P, and IF-F staining to demonstrate immunoglobulin IgA, IgG, and IgM immune deposits. Sensitivity, specificity, false-positive, and false-negative values were calculated. Results: IP-P showed sensitivity of 61.8%, 74.2%, and 64.2%, and specificity of 84.8%, 69.2%, and 66.7% for IgA, IgG, and IgM, respectively. IF-P showed a sensitivity of 45.6%, 69.4% and 52.8%, and specificity of 87.9%, 87.2% and 77.1% for IgA, IgG and IgM, respectively. False-positive cases of IF-P and IP-P were 4, 5, and 11 and 5, 12, and 16 for IgA, IgG, and IgM, respectively. Conclusion: Where IF-F lacks glomeruli or fresh renal biopsies are not available, IP-P is a sensitive method, whereas IF-P is a specific method for the evaluation of immune deposits in the renal tissue biopsies. The presence of false-positive cases in both methods deserves further research.

How to cite this article:
Alwahaibi NY, Alsidiri RM, Alsinawi TA, Almalki WH, Alsinawi SS, Alriyami MA. Immunoperoxidase and immunofluorescence on formalin-fixed, paraffin-embedded tissue sections versus immunofluorescence on frozen sections in the assessment of renal biopsies.Indian J Nephrol 2020;30:8-13

How to cite this URL:
Alwahaibi NY, Alsidiri RM, Alsinawi TA, Almalki WH, Alsinawi SS, Alriyami MA. Immunoperoxidase and immunofluorescence on formalin-fixed, paraffin-embedded tissue sections versus immunofluorescence on frozen sections in the assessment of renal biopsies. Indian J Nephrol [serial online] 2020 [cited 2020 Apr 9 ];30:8-13
Available from: http://www.indianjnephrol.org/article.asp?issn=0971-4065;year=2020;volume=30;issue=1;spage=8;epage=13;aulast=Alwahaibi;type=0