Indian J Nephrol Close
 

Figure 1: The Luminex crossmatch protocol: (1) Test beads coated with antibodies that capture donor human leukocyte antigen (HLA) antigens are incubated with the lysate prepared from donor lymphocytes (2) the class 1 and class 2 beads are coated with the respective antigens (3) subsequently the patient's serum is added and at this point of time donor specific anti HLA antibodies if present will attach to the corresponding antigens (4) this reaction is tagged using fluorescent antihuman globulin (5) the reaction is read on a dual laser flow system and quantified in terms of mean fluorescent intensity

Figure 1: The Luminex crossmatch protocol: (1) Test beads coated with antibodies that capture donor human leukocyte antigen (HLA) antigens are incubated with the lysate prepared from donor lymphocytes (2) the class 1 and class 2 beads are coated with the respective antigens (3) subsequently the patient's serum is added and at this point of time donor specific anti HLA antibodies if present will attach to the corresponding antigens (4) this reaction is tagged using fluorescent antihuman globulin (5) the reaction is read on a dual laser flow system and quantified in terms of mean fluorescent intensity